| [ sciEncE ] in KIDS 글 쓴 이(By): guest (guest) <ubppp234-211.dia> 날 짜 (Date): 2001년 12월 30일 일요일 오후 12시 28분 32초 제 목(Title): Re: simple questions on biology that's the basis of nmr . carbon-13 as well as hydrogen-1 can be used to detect the relative positions of each element. (nuclear magnetic resonance of hydrogen or carbon) so youu can't use any solvent which has h-1 or carbon-13 moeities respectively. ( for example , you can used deuterized solvent such as CD3Cl instead of CH3Cl. and you can even use hydrophilic solvent depending on the nature of the solute, which means it doesn't really matter whether solvent environment is hydrophilic or hydrophobic. if your specimen is protein which is hydrophilic, then you may need to used hydrophilic solvent. if your specimen is organic compound, say benzene, then you need to use hydrophobic solvent to dissolve it.) the reason that there is little difference of structural analysis between crystallography and nmr is, as i heard, that when you prepare the crystal for x-ray, you crystallize it in quite similar environment as its in vivo condition. that way you can minimize the errors. ( proteins dissolved in hydrophilic solvent are used to make their crystals.) in protein folding, the most important interaction in making intac protein in hydrophilic environment is van der waals force, not hydrophilic interactions. ( it is a big driving force to make protein folded..) |