| [ KAIST ] in KIDS 글 쓴 이(By): caught (꿈의극장) 날 짜 (Date): 2002년 10월 12일 토요일 오후 04시 47분 16초 제 목(Title): Re: 學士회사원’이 노벨화학상 탔다 다나카상의 업적은 MALDi나 DIOS기법등의 기초가 될만한 SLD(soft laser desorption)법의 개발이네요. 아래 article은 노벨싸이트에서 퍼왔습니다. http://www.nobel.se/ At the same time exciting things were happening in another part of the world. At the Japanese Shimadzu instrument company in Kyoto, a young Japanese engineer, Koichi Tanaka, reported an entirely different technique for the first critical stage. At a symposium in 1987 and a year later in print, Tanaka showed that the protein molecules could be ionised using soft laser desorption (SLD). A laser pulse strikes the sample which, unlike in the spray method, is in a solid or viscous phase. When the sample takes up the energy from the laser pulse it is "blasted" into small bits. The molecules let go of one another, released as intact hovering molecule ions with low charge which are then accelerated by an electrical field and detected as described above by recording their time of flight. Tanaka was the first to demonstrate the applicability of laser technology to biological macromolecules. The principle is fundamental for many of today's powerful laser desorption methods, particularly the one abbreviated MALDI (Matrix-Assisted Laser Desorption Ionisation) but also SELDI (Surface Enhanced Laser Desorption Ionisation) and DIOS (Direct Ionisation on Silicon). |